Volume 6, Issue 2, June 2018, Page: 54-59
Comparison of Antimicrobial Activities of Silver Nanoparticles Biosynthesized from Some Citrus Species
Tenderwealth Clement Jackson, Department of Pharmaceutics and Pharmaceutical Technology, University of Uyo, Uyo, Nigeria
Timma Otobong Uwah, Department of Pharmaceutics and Pharmaceutical Technology, University of Uyo, Uyo, Nigeria
Nonye Linda Ifekpolugo, Department of Pharmaceutics and Pharmaceutical Technology, University of Uyo, Uyo, Nigeria
Nsikak Anthony Emmanuel, Department of Pharmaceutics and Pharmaceutical Technology, University of Uyo, Uyo, Nigeria
Received: May 18, 2018;       Accepted: Jun. 14, 2018;       Published: Jul. 7, 2018
DOI: 10.11648/j.nano.20180602.12      View  600      Downloads  44
Abstract
Synthesis of nanoparticles was done by green method. Silver nitrate was used as the silver precursor, while the fruit juices of the citrus fruits (Citrus sinensis, Citrus limetta, Citrus aurantifolia, Citrus paradisi) were used as reducing and stabilizing agents. The nanoparticle formation was confirmed with the visible colour change from colourless to characteristic reddish brown. the surface plasmon resonance peak was at 451 and 452 nm for the silver nanoparticles The antimicrobial activities of these nanoparticles were studied against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus subtilis. Generally, MIC values of the samples against the microorganism tested ranged from 25-100mg/ml. Pseudomonas aeruginosa was the most sensitive (while Staphylococcus aureus and Bacillus subtilis were the least sensitive to the silver nanoparticles).
Keywords
Silver Nanoparticles, Green Method, Characterization, Antimicrobial
To cite this article
Tenderwealth Clement Jackson, Timma Otobong Uwah, Nonye Linda Ifekpolugo, Nsikak Anthony Emmanuel, Comparison of Antimicrobial Activities of Silver Nanoparticles Biosynthesized from Some Citrus Species, American Journal of Nano Research and Applications. Vol. 6, No. 2, 2018, pp. 54-59. doi: 10.11648/j.nano.20180602.12
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Roy S, Das T. K., (2015). Plant mediated green synthesis of silver nanoparticles. A Review. Int. J. Plant Biol. Res. 3(3):1044-1055.
[2]
Sathishkumar M, Sneha K, Won S. W., Cho C. W., Kim S, Yun Y. S. (2009). Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids Surf. B. Biointerfaces 73(2):332-338.
[3]
Sachin S, Anupama P, Meenal K (2012). Biosynthesis of silver nanoparticles by marine bacterium, Idiomarine Sp. PR-58-5. Bull. Mater. Sci. 35 (7): 1201-1205.
[4]
Rao B, Tang R. C., (2017). Green synthesis of silver nanoparticles using aqueous Eriobotyra japonica leaf extract. Adv. Nat. Sci. Nanosci. Nanotechnol. 8(2017) 015014 (8p).
[5]
Kumar P, Suranjit P, Darshit P, Patel P, Selvaraj K, Prasad S (2011). Biogenic synthesis of silver nanoparticles using Nicotiana tobaccum leaf extract and study of their antibacterial effect. Afr. J. Biotechnol. 10(41):8122-8130.
[6]
Xin Y, Qingbiao L. I., Huixuan W, Jiale H, Liqin L, Wenta W, Daohua S, Yuanbo S, James B, Luwei H, Yuanpeg W, Ning H, Lishan J (2010). Green synthesis of palladium nanoparticles using broth of Cinnamomum camphora leaf. J. Nanopart. Res. 12: 1589-1598.
[7]
Jackson T. C., Agboke A, Jackson I, Ekpuk E (2016). Biosynthesis of silver nanoparticles using Murraya koenigii and Acacia Gum. Int. J. Nanomed. 8: 1507-1516.
[8]
Prabhu N., Raj T., Gowri D., Yamuna K,. Ayisha S., Joseph S., and Puspha D (2010) Synthesis of silver phyto nanoparticles and their antibacterial efficacy. Dig. J. Nanomat. Bios; 5(1); 185.
[9]
Vivekanandhan S, Misra M, Mohanty AK (2010) Novel Glycine Max (soyabean) leaf extract based biological process for the functionalization of carbon nanotubes with silver nanoparticles. Nanosci. Nanotechnol. let 2(3): 240.
[10]
Hugund BS, Dhulappanavar GR, Ayachit NH (2015) Comparative Evaluation of Antibacterial Activity of Silver Nanoparticles Biosynthesized using Fruit Juices. J Nanomed Nanotechnol 2015, 6:271. doi:10.4172/2157-7439.1000271
[11]
CLSI, Performance Standards for Antimicrobial Disk Susceptibility Tests, Approved Standard, 7th Edition; CLSI document MO2-A11. Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087, USA, 2012.
[12]
Vijayakumar, M., et al. (2013) Biosynthesis, Characterisation and Anti-bacterial Effect of Plant-Mediated Silver Nanoparticlles Using Artemisia nilagirica. Industrial Crops and Products, 41, 235-240.
[13]
Alzahrani, E. and Welham, K. (2014) Optimization Preparation of the Biosynthesis of Silver Nanoparticles Using Watermelon and Study of Its Antibacterial Activity. International Journal of Basic and Applied Sciences, 3, 392-400.
[14]
Alzahrani, E. (2015) Ecofriendly Production of Silver Nanoparticles from Peel of Tangerine for Degradation of Dye. World Journal of Nano Science and Engineering, 5, 10-16.
[15]
Sudha, M., Murugan, B. (2015) Orange Seed Mediated Green Synthesis Silver Nanoparticles. International Journal of Biosciences and Nanosciences. Volume 2 (11), 2015, pp. 215-220.
Browse journals by subject