Volume 7, Issue 2, June 2019, Page: 11-20
Fabrication of Electrospun Polycaprolactone/Gelatin Composite Nanofibrous Scaffolds with Cellular Responses
Jaianand Kannaiyan, CelluGen Biotech Private Limited, Research and Development, Gurgaon, Haryana, India
Saurabh Khare, CelluGen Biotech Private Limited, Research and Development, Gurgaon, Haryana, India
Suriya Narayanan, CelluGen Biotech Private Limited, Research and Development, Gurgaon, Haryana, India
Firdosh Mahuvawalla, CelluGen Biotech Private Limited, Research and Development, Gurgaon, Haryana, India
Received: Oct. 9, 2019;       Accepted: Nov. 4, 2019;       Published: Nov. 8, 2019
DOI: 10.11648/j.nano.20190702.11      View  37      Downloads  17
Abstract
Numerous wound care products have been investigated for skin tissue engineering. Factors which influence to skin substitute selection are condition of patient, wound depth, infection in wound. Other factors which also play role in deciding skin substitute are cost, its availability, ease of storage, required operative interventions. With perspective of Indian market, commercial available skin substitutes are either costly or their availability restricted to major cities only. A cost effective skin substitute is strongly needed to heal wounds with minimal scarring and maximum function. The aim of this study was to investigate the possibility of synthetic scaffold loaded with Wharton’s jelly derived Mesenchymal stem cells and to access the role of scaffolds in proliferation and differentiation of MSCs in-vitro, in order to achieve for the healing of wound graft substitutes with improved biological properties. As a result, WJ-MSCs were isolated, harvested and seeded on the surface of the fabricated PCL/GE nanofibrous scaffold. The biological properties and growth of MSCs were studied for anti-inflammation, cytotoxicity, cell proliferation, and SEM analysis indicated that the fabricated synthetic scaffold supported cells attachment, viability, and proliferation of cells. The characterization studies of nanofibers were studied for ATR-FTIR, XRD, TEM, viscosity, and degradation studies suggest that the nanofibrous scaffold loaded with stem cells could be an excellent tissue-engineered skin base for wound healing and skin regeneration.
Keywords
Electrospinning, Polycaprolactone (PCL), Gelatin (GE), Nanofibrous Scaffold, Mesenchymal Stem Cells (MSCs)
To cite this article
Jaianand Kannaiyan, Saurabh Khare, Suriya Narayanan, Firdosh Mahuvawalla, Fabrication of Electrospun Polycaprolactone/Gelatin Composite Nanofibrous Scaffolds with Cellular Responses, American Journal of Nano Research and Applications. Vol. 7, No. 2, 2019, pp. 11-20. doi: 10.11648/j.nano.20190702.11
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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